BioPHP 46 is an open source project consisting of PHP classes, functions and minitools. The classes include sequence, sequence database, sequence alignment, and restriction enzyme. Making use of the classes and functions is a matter of downloading them and including them using an include statement within your PHP script. The documentation is minimal. It is mainly a matter of reading source code comments.
The minitools include sequence manipulation, melting temperature, finding palindromic sequences, alignment, restriction digest, translation, microarray analysis, protein sequence information, and oligonucleotide frequency. The minitools include web interfaces, which is a very convenient and can save time in developing your own or, at least, provide a head start. Also, the minitools have demos that are run from the biophp.org server. Programmatic use of the minitools is by copying and editing.
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